To investigate the interactions between SO(2) and B(a)P, male Wistar rats were exposed to intratracheally instilled with B(a)P or SO(2) inhalation alone or together. We detected mRNA expression of CYP1A1 and 1A2, 7-ethoxyresorufin O-deethylase (EROD), JAK pathway and methoxyresorufin O-demethylase (MROD) activities in livers. The mRNA and protein levels of several cancer-related genes were analyzed in livers by real-time RT-PCR and Western blot, respectively. The EROD/MROD activities and CYP1A1/2

expression were down-regulated by SO(2) but up-regulated by B(a)P alone. Exposure of SO(2) alone induced c-fos, c-jun, c-myc, H-ras, and p53 expression, and depressed p16 and Rb expression in livers. The effects of B(a)P on the above gene were similar to SO(2) except c-fos expression. Furthermore, SO(2) + B(a)P exposure increased the expression of c-fos, c-jun, c-myc, and p53, and decreased p16 and Rb expression in livers compared with exposed to SO(2) or B(a)P alone. However, no synergistic effects

were observed BAY 73-4506 on H-ras and CYP1A1/2 after SO(2) + B(a)P exposure. Our findings indicate that multiple cell cycle regulatory proteins play key roles in the toxicity of SO(2) and B(a)P in livers. It might involve the activation of c-fos, c-jun, c-myc, and p53. And p16-Rb pathway might also participate in the progress. Although the gene products we studied are classed as oncogenes and tumor suppressor genes, their functions actually relate to more general processes of control of cell proliferation, survival, and/or apoptosis. (C) 2009 Wiley Periodicals, Inc. Environ Toxicol 25: 169-179, 2010.”
“Long

spinal deformity fusions in elderly patients continue to be controversial. However, there is a PFTα molecular weight growing population of elderly patients with spinal deformities that may be optimally treated by reconstructive surgery requiring a long fusion to the sacrum. This study evaluated clinical outcomes in elderly (> 65) adult deformity patients who underwent posterior instrumented reconstruction consisting of fusion from the thoracic spine to the sacrum with iliac fixation.

Patients in a prospective database for adult spinal deformity who had a posterior reconstruction with an instrumented fusion from the thoracic spine to the sacrum that included iliac fixation with minimum 2-year follow-up were identified. Two cohorts were compared: patients 65 years and older and patients 55 years and younger. Student’s t test for independent groups was used to determine any significant differences between continuous variables. Chi-square was used to compare categorical demographic variables between the two groups.

The 65 and older group consisted of 15 patients with an average age of 71 years (range 65-78 years). The 55 and younger group consisted of 25 patients with an average age of 45 years (range 30-55 years). The older group had a worse mean co-morbidity score (4.6 vs.