The following compounds were tested, but do not support growth: L-arginine, butanol, citrate, ethanol, formate, D-fructose, D-glucose, glycerol, glycolate, DL-lactate, methanol, 2-oxoglutarate, L-phenylalanine, L-serine and sucrose. Thiosulfate does not stimulate growth. The major cellular fatty acids upon culturing

on plates of Marine Agar under fully aerobic conditions EPZ015938 mouse are C16:1ω7c, C17:1ω8c, C18:1ω7c, C16:0, C15:0, C17:1ω6c, and C17:0. Methods Source of sample and isolation procedure The general isolation procedure has been Vorinostat chemical structure already described in a previous report [25], which was however focused mainly on the isolation of Rhodopirellula strains. In brief, the OM60/NOR5 isolates were obtained as follows: In October 2005 sediment samples were collected from a tidal flat area at Königshafen bay, near the town of List on the German Island of Sylt. The approx. geographic coordinates of the sampling site were 55.04° North CRT0066101 mw and 8.42°

East. Most samples were obtained from the top oxic layer of muddy or sandy intertidal sediments. After transportation to the laboratory additional 1:10 and 1:100 dilutions of the original sediment samples were prepared in artificial seawater, then 50 or 200 μl aliquots of each sample were spread on agar plates Phosphatidylethanolamine N-methyltransferase of Pla-rich medium supplemented with the antibiotics ampicillin and cycloheximide added in a concentration of 2.0 g/l each. The exact composition of Pla-rich medium has already been described elsewhere [25]; essentially it is composed

of artificial seawater supplemented with vitamins and trace elements that contains 0.25 g/l each of yeast extract, peptone and glucose as substrates. Colonies displaying a pinkish to red-violet pigmentation appeared after several weeks of incubation at 24°C. Pigmented colonies were further purified by subsequent transfers on Pla-rich agar plates without antibiotics. To determine purity and the phylogenetic affiliation of isolated strains the 16S rRNA genes were PCR-amplified from whole cells and then directly sequenced using an ABI 3130xl DNA sequencer (Applied Biosystems; Darmstadt, Germany). A total of 240 red-pigmented colonies were obtained, of which 22 could be affiliated to the OM60/NOR5 clade by phylogenetic analyses of their partial 16S rRNA gene sequences.