The aim of this study is to investigate whether DFX has any effects on the development of liver fibrosis and preneoplastic lesions in animal model. In vitro)We examined cell growth by MTS assay and apoptosis by Caspase

3 activity using human hepatoma cell(HepG2,HuH7,Hep3B).In vivo) 1 )The effects of DFX were examined using the choline-defi-cient L-amino acid-defined (CDAA) diet-induced rat liver fibrosis model.The total study periods were 16,and20weeks.One group received CDAA diet with DFX(20mg/kg/adult),The other was CDAA diet only.Liver fibrosis was analyzed by Azan,Sirius-red,aSMAexpression and hydroxyproline level.The preneoplastic lesion was assessed by glutathione S-transferase placental form(GST-P) expression.The

change of laboratory data was analyzed.Type ICG-001 clinical trial I procollagen,TIMP1 ,2,TGFb mRNA were analyzed using both Real time-PCR and DNA array.2)We examined the effects of DFX using N-nitrosodiethylni-trosamine(DEN)-induced liver cancer C59 wnt datasheet mouse model. One group received with DFX(20mg/kg/adult) from 5 months to 8 months after DEN injection of 1 mg/kg at 14 days,The other was DEN injection only.Liver cancer was analyzed by HE,AFP,PCNA,CD44 expression.The oxidative stress was analyzed by 4HNE,8OHdG expression.We compared many gene expressions of cancer and non cancer tissues between DFX group and control. The cell growth of hepatoma cells was inhibited with DFX in a dose-dependent manner(P<0.01).The caspase3 activity was increased with DFX in a dose-dependent manner(P<0.01).In CDAA model,DFX prevented liver fibrosis by Azan,Sirius-red,aSMA expression(p<0.05)and hepatic hydroxyproline level was decreased.DFX reduced both the area and numbers of GST-P positive preneoplastic lesions(p<0.01).Administration

of DFX reduced levels of 4HNE (DFX 3.3,CDAA Dichloromethane dehalogenase only 8.0,p<0.01), 8OHdG (DFX 1.3,CDAA only 2.0 ng/ugDNA,p<0.05).DFX inhibited Type 1 procollagen,TIMP1 ,2 mRNA expression (all of p<0.01).In DEN model, DFX reduced both the area and numbers of tumor lesions (p<0.01).DFX prevented AFP,CD44 expression (p<0.05)and significantly reduced levels of 4HNE,8OHdG. Our results indicated that DFX inhibited liver fibrosis and preneoplastic lesions. Deferasirox may be the new drug for Liver Fibrosis and Hepatocellular Carcinoma. Disclosures: The following people have nothing to disclose: Naoki Yamamoto, Takahiro Yamasaki, Koichi Uchida, Norikazu Tanabe, Taro Takami, Issei Saeki, Koichi Fuji-sawa, Masaki Maeda, Shuji Terai, Isao Sakaida Background: Forkhead box M1 (FOXM1) transcription factor plays an important role in hepatocarcinogenensis.