56. Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007, 24:1596–1599.PubMedCrossRef selleck screening library 57. Carver T, Berriman M, Tivey A, Patel C, Böhme U, Barrell BG, Parkhill J, Rajandream MA: Artemis and ACT: viewing, annotating and comparing sequences stored in a relational database. Bioinformatics 2008, 24:2672–2676.PubMedCrossRef 58. Church GM, Gilbert W: Genomic sequencing. Proc Natl Acad Sci USA 1984, 81:1991–1995.PubMedCrossRef 59. Brenner DJ, Farmer JJ: Enterobacteriales. In Bergey’s Manual of Systematic

Bacteriology. Volume 2. Edited by: Brenner D, Krieg NR, Staley JT, Garrity GM. Springer; 2005:587–848. 60. Gavini F, Ferragut C, Lefebvre B, Leclerc H: E’ tude taxonomique d’ente’robacte’ries appartenant ou apparente’es au genre Enterobacter . Ann Microbiol (Paris) 1976, 127B:317–335. Authors’ contributions WR conceived the study and was involved in all stages of experimental work and data analysis and drafted the manuscript. EP participated in strain isolation and manuscript preparation. MK participated in database searches and sequence annotation. DKS interpreted the results regarding the multimer resolution sites. BP participated in data analysis and helped to draft the manuscript. All authors read and approved the BAY 11-7082 ic50 final manuscript.”
“Background Bacteriophage Φ6 was the first member of

the Cystoviridae to be isolated [1]. In 1999 we isolated a number of phages that were members of the Cystoviridae [2]. Some were close relatives of Φ6 while others were rather distantly related in that they shared little or no base sequence similarity although their gene order was similar and they all contained genomes of three segments of dsRNA enclosed in a polyhedral shell that was, in turn, encased in a lipid-containing membrane. All members of this family have an inner core composed of 120 molecules of the major structural protein P1, 12 hexamers of the

packaging NTPase P4, 12 molecules of polymerase P2 and about 30 molecules of auxilliary protein P7. The core is encased in a shell of protein P8 in all members except the Φ8 group. This is designated as the nucleocapsid. The nucleocapsid is covered by a lipid-containing PTK6 membrane which has protein P9 as its major component and proteins P6 and P3 which determine host specificity. We proposed four groups represented by phages Φ6, Φ13, Φ12 and Φ8. The phages in the last three groups attached to host cells through rough LPS while the Φ6 group attached to type IV pili. We have recently isolated a new collection of phages and they seem to fit into the previously proposed groups with some important distinctions. In this paper we describe bacteriophage Φ2954 which has similarity to Φ12 [3] in the amino acid composition of several of its proteins but whereas Φ12 QNZ supplier attaches to rough LPS, Φ2954 attaches to type IV pili.