5-Fluorouracil (5-FU) chemotherapy for cancer treatment is often accompanied by severe intestinal injury (mucositis). It is unknown whether ME PLX4032 concentration impacts on the processes of mucositis and neoplasia in normal and transformed epithelial cells. Aims: MEs from different host trees (Quercus: Oak,

Fraxini: Ash and Mali: Apple) in the presence or absence of 5-FU chemotherapy, were examined for their effects on viability of colon cancer and normal non-transformed intestinal cells in vitro. Methods: 3-(4,5-Dimethylthiazol-2 yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay was used to determine Caco-2 (colonic cancer) and IEC-6 (non-transformed) cell viability after 48 hr incubation with MEs (Quercus: Oak, Fraxini: Ash and Mali: Apple) (1–100 μg/mL)

or MEs (1–100 μg/mL) combined with 5-FU (100 μM for Caco-2 and 5 μM for IEC-6). Statistical significance was assumed at p < 0.05. Results: Fraxini; with highest levels of lectin and viscotoxin, was the most potent ME followed by Quercus and Mali with IC50 values of 42.7, 65.5 and 84.4 μg/mL, respectively, check details on Caco-2 cells. Fraxini (50 μg/mL) when combined with 5-FU (5 μM), significantly increased the toxicity of 5-FU on IEC-6 cells compared to Fraxini (50 μg/mL) alone (p < 0.05). None of the MEs, when combined with 5-FU, significantly increased 5-FU toxicity on Caco-2 cells compared to the corresponding MEs administered in the absence of 5-FU chemotherapy. Quercus and Mali did not alter the degree of 5-FU toxicity on IEC-6 cells, compared to the same concentrations

of Quercus and Fraxini without 5-FU. Conclusions: Of the ME species tested, the oak sourced ME (Quercus) demonstrated significant toxicity to colon cancer cells with lesser impact on normal intestinal epithelial cells. Future studies could investigate ME effects in models of colon cancer in vivo to determine whether ME (particularly Quercus) inhibits the development of colonic neoplasia without exacerbating the undesirable impact of 5-FU on the normal healthy intestine. SM ABIMOSLEH,1,2 CD TRAN,1,2 GS HOWARTH1,2,3 1Department of Gastroenterology, Women’s and Children’s Hospital, North Adelaide, Paclitaxel cell line South Australia, Australia, 2Discipline of Physiology, School of Medical Sciences, Faculty of Health Sciences, The University of Adelaide, Adelaide, South Australia, Australia, 3School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy Campus, Roseworthy, South Australia, Australia Introduction: Mucositis resulting from cancer chemotherapy is characterized by intestinal inflammation and ulceration. Treatment options are variably effective, highlighting the need to broaden therapeutic approaches. Previously, Emu Oil (EO) improved intestinal architecture (Br J Nutr, 2010) in a rat model of chemotherapy-induced mucositis.