Thus, this

rootstock’s ability to maintain shoot growth in replant soil may be attributable to relative Lapatinib supplier replant pathogen resistance in distal root branches as well as tolerance of infection based on rates of root growth.”
“To evaluate the efficiency of an original slow freezing protocol on the quality and function of human ovarian cortex.

Human ovarian tissues were cryopreserved using a freezing medium supplemented with propanediol and raffinose as cryoprotectants and antioxidants (L-glutamine, taurine). Samples were then frozen using a faster cooling rate than the usual one. Viability and morphology of follicles, DNA fragmentation in follicles and stroma as well as histology of the vascular endothelium were analyzed before and after freezing/thawing. Moreover, a functional analysis was performed based on the evaluation of follicular growth and development in thawed ovarian tissues that were cultured in vitro.

Our freezing/thawing see more protocol allows preservation of a high proportion of viable follicles and the preservation of the different follicle developmental stages (p > 0.05 versus fresh control). 70.5 +/- 5.2 % of follicles

retained an intact morphology after cryopreservation (p = 0.04). Stroma cells but not follicles exhibited a slight increase of DNA fragmentation after thawing (p < 0.05). Microvessel endothelium PND-1186 ic50 within thawed tissues appeared to be preserved. Granulosa cells showed signs of proliferation in follicles cultured for 12 days. Secretion of 17 beta-oestradiol significantly increased during

in vitro culture.

This protocol leads to good preservation of ovarian integrity and functionality post-thawing and thus appears as a suitable technique of ovarian tissue cryopreservation in clinical settings. Further research could be extended to optimize conditions of in vitro culture.”
“Background: High-resolution magnetic resonance (MR) imaging has been used for MR imaging-based structural stress analysis of atherosclerotic plaques. The biomechanical stress profile of stable plaques has been observed to differ from that of unstable plaques; however, the role that structural stresses play in determining plaque vulnerability remains speculative.

Methods: A total of 61 patients with previous history of symptomatic carotid artery disease underwent carotid plaque MR imaging. Plaque components of the index artery such as fibrous tissue, lipid content and plaque haemorrhage (PH) were delineated and used for finite element analysis-based maximum structural stress (M-C Stress) quantification. These patients were followed up for 2 years. The clinical end point was occurrence of an ischaemic cerebrovascular event.