Silver nanoparticles with a diameter of 40 ± 4 nm (purchased from Sigma-Aldrich, St. Louis,

MO, USA) were spiked into the bacteria-BC sample for SERS detection. Experimental system For the purpose of driving DEP forces, a multi-output function generator (FLUKE 284, FLUKE Calibration, Everett, WA, USA) with four isolation channels was used to supply an output voltage range of 0.1 to 20 Vp-p with a frequency range of 0 to 16 MHz. The experiment was observed through an inverted microscope (Olympus IX 71, Olympus Corporation, Shinjuku-ku, Japan), and a fluorescent light source was used to excite the fluorescent nanocolloids. The experimental results were recorded TPCA-1 in both video and photo formats using a high-speed charge-coupled device (CCD) camera (20 frames/s, Olympus DP 80, Olympus Corporation, Shinjuku-ku, Japan). An argon laser at 532 nm was used for excitation through an inverted microscope. The laser power at the sample position

was around 1 mW, and the scattering light was collected using a 10× objective lens connected to a CCD. The Raman shift KU55933 clinical trial was calibrated using a signal of 520 cm-1 generated from a silicon wafer. All reported spectra of the exposure time were set to 5 s, and signal was accumulated two times in a range of 500 (approximately 2,000 cm-1). Rayleigh scattering check details was blocked using a holographic notch filter, and the tilted baselines of some SERS spectra were corrected to flat using OMNIC 8 software (Thermo Fisher Scientific, Waltham, MA, USA). The integrated experimental system is shown in Figure  1. Figure 1 Experimental flow chart. (a) AgNPs were spiked and resuspended into the prepared {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| bacteria solution. (b) AC voltage was applied to separate and collect the bacteria in the middle region. The AgNPs can also be trapped with the bacteria

aggregate via the amplified positive DEP force. After bacteria-AgNP concentration and adsorption, the Raman laser was then irradiated to the bacteria-NP aggregate separated from the blood cells for the purpose of SERS identification. (c) On-chip identification of bacteria by comparing the detected SERS spectra to the spectra library. Results and discussion Finite element simulation Figure  2a,b shows the finite element simulation results for the electric field distribution without and with the microparticle assembly, respectively. The electric fields were solved numerically using finite element analysis software (Comsol Multiphysics 3.5, Comsol Ltd., Burlington, MA, USA). The electric scalar potential satisfies Poisson’s equation, and the electric field and displacement are obtained from the electric potential gradient.