Results are reported PF-02341066 order as the percentage of 100 cells analyzed. Groupwise comparison was made by the Student’s t-test, p < 0.05 was considered significant. RNA interference Two siRNAs for TfR1 (Tfrc_4 (TACCCATGACGTTGAATTGAA), and Tfrc_1 (ATCGTTAGTATCTAACATGAA)) were designed using proprietary software and synthesized. Both had 3' modifications with Alexa Fluor 555. Transfection

of macrophages was accomplished with Lipofectamine 2000 according to the manufacturer’s instruction. Only Tfrc1 had significant activity (data not shown) and was used for all further studies Real-time RT-PCR Total RNA was isolated and digested with DNAse using the Microto-Midi Total RNA Purification System (Invitrogen, catalog no. 12183-018) according to the product instructions. RNA concentrations were determined by a RiboGreen assay (Molecular Probes, Carlsbad, CA; catalog no. R11490). Primer design was performed with the eXpress Profiling Suite software (Beckman) and mRNA sequences from the GenBank database. Uniqueness and specificity of each primer was verified using the Basic Local Alignment Search Tool http://​www.​ncbi.​nlm.​nih.​gov/​blast returning Genbank accession numbers. Primers are listed in Table 1. Table 1 Primers used for real-time RT PCR Gene Accession number

Forward primer (5′ → 3′) Reverse Primer (5′ → 3′) GAPDH NM_008084 AGGTGACACTATAGAATACCCACTAACATCAAATGGGG GTACGACTCACTATAGGGACCTTCCACAATGCCAAAGTT IRP1 NM_007386 AGGTGACACTATAGAATAACTTTGAAAGCTGCCTTGGA GTACGACTCACTATAGGGACTCCACTTCCAGGAGACAGG almost IRP2 NM_022655 AGGTGACACTATAGAATATGAAGAAACGGACCTGCTCT GTACGACTCACTATAGGGAGCTCACATCCAACCACCTCT TfR1 BC054522 AGGTGACACTATAGAATATGCAGAAAAGGTTGCAAATG GTACGACTCACTATAGGGATGAGCATGTCCAAAGAGTGC GSK1210151A purchase Dmt1 NM_008732 AGGTGACACTATAGAATAGCCAGCCAGTAAGTTCAAGG GTACGACTCACTATAGGGAGCTGTCCAGGAAGACCTGAG LcnR NM_021551 AGGTGACACTATAGAATAGCAAGGCTACCCCATACAAA GTACGACTCACTATAGGGAAAGAGCGAGGTCTGGGAAAT

Lcn2 NM_008491 AGGTGACACTATAGAATACTGAATGGGTGGTGAGTGTG GTACGACTCACTATAGGGATATTCAGCAGAAAGGGGACG Steap3 BC037435 AGGTGACACTATAGAATACTCTCTGTGCAGTCTCGCTG GTACGACTCACTATAGGGATGCAGAGATGACGTTGAAGG Hmox1 NM_010442 AGGTGACACTATAGAATACCTCACTGGCAGGAAATCAT GTACGACTCACTATAGGGACCAGAGTGTTCATTCGAGCA Fpn1 AF226613 AGGTGACACTATAGAATATGCCTTAGTTGTCCTTTGGG GTACGACTCACTATAGGGAGTGGAGAGAGAGTGGCCAAG Hamp1 NM_032541 {Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|buy Anti-diabetic Compound Library|Anti-diabetic Compound Library ic50|Anti-diabetic Compound Library price|Anti-diabetic Compound Library cost|Anti-diabetic Compound Library solubility dmso|Anti-diabetic Compound Library purchase|Anti-diabetic Compound Library manufacturer|Anti-diabetic Compound Library research buy|Anti-diabetic Compound Library order|Anti-diabetic Compound Library mouse|Anti-diabetic Compound Library chemical structure|Anti-diabetic Compound Library mw|Anti-diabetic Compound Library molecular weight|Anti-diabetic Compound Library datasheet|Anti-diabetic Compound Library supplier|Anti-diabetic Compound Library in vitro|Anti-diabetic Compound Library cell line|Anti-diabetic Compound Library concentration|Anti-diabetic Compound Library nmr|Anti-diabetic Compound Library in vivo|Anti-diabetic Compound Library clinical trial|Anti-diabetic Compound Library cell assay|Anti-diabetic Compound Library screening|Anti-diabetic Compound Library high throughput|buy Antidiabetic Compound Library|Antidiabetic Compound Library ic50|Antidiabetic Compound Library price|Antidiabetic Compound Library cost|Antidiabetic Compound Library solubility dmso|Antidiabetic Compound Library purchase|Antidiabetic Compound Library manufacturer|Antidiabetic Compound Library research buy|Antidiabetic Compound Library order|Antidiabetic Compound Library chemical structure|Antidiabetic Compound Library datasheet|Antidiabetic Compound Library supplier|Antidiabetic Compound Library in vitro|Antidiabetic Compound Library cell line|Antidiabetic Compound Library concentration|Antidiabetic Compound Library clinical trial|Antidiabetic Compound Library cell assay|Antidiabetic Compound Library screening|Antidiabetic Compound Library high throughput|Anti-diabetic Compound high throughput screening| AGGTGACACTATAGAATAGAGAGACACCAACTTCCCCA GTACGACTCACTATAGGGATCAGGATGTGGCTCTAGGCT Ftl1 NM_010240 AGGTGACACTATAGAATAAAGATGGGCAACCATCTGAC GTACGACTCACTATAGGGAGCCTCCTAGTCGTGCTTGAG Fth1 NM_010239 AGGTGACACTATAGAATACTCATGAGGAGAGGGAGCAT GTACGACTCACTATAGGGAGTGCACACTCCATTGCATTC The reverse transcription reactions were carried out with 20 units of Moloney Murine Leukemia Virus (MMuLV) reverse transcriptase (Fisher Scientific, catalog no. BP3208-1), 20 units RNase inhibitor (Fisher Scientific, catalog no. BP3225-1), RT-PCR buffer containing 10 mM Tris-HCl and 50 mM KCl; 2.5 mM MgCl2; 10 mM dithiothreitol; and 1 mM of each dNTP. The concentration of each reverse primer was 5 μM.