Our results suggest that claudin-2 may play an important role in enabling breast cancer cells to metastasize to the liver. Poster No. 34 Metastasis Genes Expression Profile in Cholangiocarcinoma Cell Induced by External Estrogenic Agent in associate with TFF1 Trefoil Protein Peti Thuwajit 1,2,3 , Chanitra Thuwajit1,2,3 1 Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, 2 Division of Medical Molecular Biology, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, 3 Liver Fluke
and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand Cholangiocarcinoma is the carcinoma generated from bile duct epithelium. The prevalence of cholangiocarcinoma is low among worldwide, however it was raised each year. In Thailand cholangiocarcinoma selleck kinase inhibitor is endemic especially in northeastern part and associated with a liver fluke Smoothened Agonist Opisthorchis viverrini infection. The prognosis of cholangiocarcinoma is quite poor because it has high metastasis rate. Previous study this website showed that cholangiocarcinoma had impairment of estrogen metabolizing enzyme that could leading to the accumulation of
estrogen in plasma as we found in our preliminary study. Estrogen itself could induce tumor progression include tumor growth and invasion. TFF1 trefoil protein, an estrogen responsive protein, is a secreted protein that has motogenic effect and can promote cell migration and invasion. In this study we tested the effects of 17b-estradiol, the most potent
natural estrogenic substance, on invasion and metastasis genes expression of cholangiocarcinoma cell lines in vitro. To test the role of TFF1 trefoil protein in estrogen-stimulated invasion, the permanent Methocarbamol knockdown cholangiocarcinoma cell line and mock cell were generated and treated with 17b-estradiol. The results showed that 17b-estradiol could stimulate the invasion of cholangiocarcinoma cell but not in TFF1 knockdown cell compared to both negative control and mock control. Eighty-four tumor metastasis genes expression of estrogen treated cholangiocarcinoma cells (normal control, mock and TFF1 knockdown cell) was measured by RT2 ProlifilerTM PCR array system. By compared between 3 cell groups, the result indicated 14 genes (CHD4, COL4A2, CST7, CTBP1, KISS1R, IL18, MET, MMP10, NF2, NME1, PTEN, TIMP2, TIMP4 and TRPM1) associated with invasive property induced by estrogen and TFF1 trefoil protein. The pathway of estrogen induced metastasis genes should be analyzed and the results should indicate the mechanism and control of cholangiocarcinoma metastasis for development of new therapeutic method. Poster No.