One study investigated the use of infiltrating macrophages to deliver a systemically #Ribociclib manufacturer randurls[1|1|,|CHEM1|]# administered gene therapy in stroke [106]. Plasmids expressing enhanced green fluorescent protein (EGFP) and fibroblast growth factor-2 (FGF-2) were complexed

with cationic liposomes, administered into the femoral vein resulting in expression of EGFP Inhibitors,research,lifescience,medical and FGF-2 in infiltrating macrophages and in the cerebral infarction. 4.5. Other There has also been some attention paid to “Trojan monocytes” for drug delivery to the brain [107] as a means of delivering drugs to inaccessible sites (Figure 1). Delivery of drugs to the brain is greatly hampered by the extremely selective permeability of the blood brain barrier (BBB). However, immune cells such as phagocytes can cross this barrier. Therefore by targeting circulating mononuclear cells with drug-loaded liposomes, this natural

BBB uptake process can be harnessed for drug delivery. Previous studies have used RGD-liposomes Inhibitors,research,lifescience,medical [29, 60, 61] as well as magnetic liposome formulations [29, 108] for delivery to the Inhibitors,research,lifescience,medical brain via monocytes and neutrophils. Afergan et al. prepared PG-composed liposomes for the delivery of the neurotransmitter serotonin [109]. In vivo studies showed localisation to the brain to be improved by liposome encapsulation and that the delivered liposomes Inhibitors,research,lifescience,medical were intact. FACS analysis of rabbit blood 4 hours posttreatment showed higher uptake of liposomes by monocytes over granulocytes. Uptake was also observed by monocytes and neutrophils in vivo and in vitro but it was shown that monocytes were the neurodelivery

cells by an alendronate monocyte depletion study [109]. More recently Saiyed et al. developed azidothymidine 5′-triphosphate (AZTTP) containing magnetic liposomes as a therapeutic for neuroAIDS [108]. Magnetic nanoparticles (Fe3O4, magnetite) were encapsulated with AZTTP in neutral liposomes, and transmigration of the liposomes in monocytes was monitored across an in vitro BBB model in Inhibitors,research,lifescience,medical the presence of a magnet. By about magnetic liposome endocytosis, monocytes become magnetic and responded to magnetic fields [29]. The transmigration of magnetic monocytes was significantly increased in the presence of a magnet in comparison to nonmagnetic monocytes. A study by Matsui et al. examined the potential of peripheral blood monocytes (PBMCs) and human peritoneal macrophages as drug carriers in gastric cancer [36]. Oligomannose-coated liposomes were successfully targeted to monocytes and macrophages showing significantly higher uptake than bare liposomes. These liposome-loaded human monocytes and macrophages were found to accumulate at the disease target site micrometastases and milky spots of the omentum in mice and ex vivo in resected human omentum. 5.