\n\nMethods – We examined the expression of endogenous markers of mitotic activity, proliferating cell nuclear antigen, and vimentin as a marker for neuronal progenitor cells, if any, in the adult rat cortex after spreading depression stimulation. Immunohistochemical analysis JIB-04 was also performed using antibodies for proliferating cell nuclear antigen, for vimentin, and for nestin. Nestin is a marker for activity dividing neural precursors.\n\nResults – At the end of spreading depression (Day 0), glial fibrillary acidic protein-positive cells in the subpial zone and cortical Layer I demonstrated increased mitotic activity, expressing vimentin and nestin.
On Day 1, nestin(+) cells were found spreading in deeper cortical layers. On Day 3, vimentin(-)/nestin(+), neural precursor-like cells appeared in cortical Layers V to VI. On Day 6, new immature neurons appeared in cortical Layers V to VI. Induced spreading selleck chemical depression evokes cell division of astrocytes residing in the subpial zone, generating neural precursor-like cells.\n\nConclusions – Although neural precursor-like cells found in cortical Layers V to VI might have been transferred from the germinative zone rather than the cortical subpial zone, astrocytic cells in the subpial zone may be potent neural progenitors that can help to reconstruct impaired central nervous system tissue. Special caution is required
when observing or treating spreading depression waves accompanying pathological conditions in the brain. (Stroke. 2009; 40: e606-e613.)”
“Purpose: To test the hypothesis that radiation dose to F-18-fluorodeoxyglucose positron emission tomography (F-18-FDG-PET)-defined active bone marrow (BMACT) subregions is correlated with hematologic toxicity in cervical cancer patients treated with chemoradiotherapy.\n\nMethods and Materials: The conditions of 26 women with cervical cancer who underwent F-18-FDG-PET before treatment with concurrent cisplatin and intensity-modulated radiation therapy
were analyzed. BMACT was defined as the subregion of total bone marrow (BMTOT) with a standardized uptake CA3 ic50 value (SUV) equal to or above the mean for that individual. Inactive bone marrow (BMINACT) was defined as BMTOT – BMACT. Generalized linear modeling was used to test the correlation between BMACT and BMINACT dose-volume metrics and hematologic nadirs, particularly white blood cell count (WBC) and absolute neutrophil count (ANC).\n\nResults: Increased BMACT mean dose was significantly associated with decreased log(WBC) nadir (beta = -0.04; 95% CI, -0.07to -0.01; p = 0.009), decreased log(ANC) nadir (beta = -0.05; 95% CI, -0.08 to -0.02; p = 0.006), decreased hemoglobin nadir (beta = -0.16; 95% CI, 0.27 to 0.05; p = 0.010), and decreased platelet nadir (beta = 6.16; 95% CI, 9.37 to -2.96; p < 0.001). By contrast, there was no association between BMINACT mean dose and log(WBC) nadir (beta = -0.01; 95% CI, -0.