\n\nMethods: Ten experimental adhesive systems were formulated

according to the addition of CHX diacetate (0 [control], 0.01, 0.05, 0.1 and 0.2%) in the two ER. For UTS and DC, specimens were constructed and tested after 24 h. For WS, SO and CR, after specimens build-up, they were stored in water and the properties measured after 60 days. The occlusal enamel of fifty molars was removed and the adhesives were applied in dentine surface after 37% phosphoric acid etching. After composite resin build-ups, specimens were longitudinally sectioned to obtain resin-dentine bonded sticks (0.8 mm(2)). Specimens were tested in tension at 0.5 mm/min in the IM or 1Y. For NL, 2 bonded selleck kinase inhibitor sticks from each tooth were prepared and analyzed under SEM. The data were submitted

to appropriate statistical analysis (alpha = 0.05).\n\nResults: The addition of CHX did not influence UTS, DC, WS and SO (p < 0.05). Higher CR was observed in adhesives with higher concentration of CHX (p < 0.05). After 1Y, significant reductions of mu TBS and increases of NL were observed in the control groups (p < 0.05). Reductions of mu TBS and increase of NL over time were not observed (AM) for CHX-containing adhesives or it was less pronounced than the control (XP) regardless of the CHX concentration.\n\nConclusions: The addition of CHX diacetate in concentrations until 0.2% in the simplified ER adhesive systems may be an alternative to increase the long-term stability of resin-dentine interfaces,

without SNX-5422 nmr jeopardizing the adhesives’ mechanical properties evaluated. (C) 2013 Published by Elsevier Ltd.”
“Only a limited number of noninvasive techniques are available to directly measure the dynamic behavior of lipids in model and cell membranes. Here, we explored whether a commercial instrument could be used for fluorescence correlation spectroscopy (FCS) under pulsed stimulated emission depletion (STED). To overcome issues with photobleaching and poor distinction between confocal and STED signals, we implemented resonant line-scan STED with filtered FCS, which has the additional benefit of autocalibrating the dimensions of the point-spread IPI-145 Angiogenesis inhibitor function and obtaining spatially resolved molecular mobility at subdiffraction resolution. With supported lipid bilayers, we achieved a detection spot radius of 40 nm, although at the expense of decreased molecular brightness. We also used this approach to map the dynamics of Atto646N-labeled sphingomyelin and phosphatidylethanolamine in the plasma membrane. Despite the reliability of the method and the demonstration that photobleaching and the photophysical properties of the dye did not influence diffusion measurements, we found great heterogeneities even within one cell. For both lipids, regions of high local density correlated with slow molecular diffusion, indicating trapping of Atto646N-labeled lipids. Future studies with new dyes are needed to reveal the origin of the trapping.