In total, 160 male birds at ages of 5 and 6 wk Epigenetics inhibitor were exposed to temperatures of -9 to -15 degrees C (cold stressed) and +20 degrees C (control) in a simulated transport chamber for 3 h before slaughter followed by 0 or 2 h of lairage. Bird physiology parameters, including core body temperature, live shrink, blood glucose, and muscle temperature, were assessed. Core body temperature was monitored every minute using i-Button data loggers, and live shrink and blood

glucose were assessed. Total glucose and lactate concentrations at 30 h postmortem, as well as ultimate pH (pH(u)), color, and water-holding attributes were evaluated on pectoralis major muscle of breast and iliotibi-alis muscle of thigh. Birds were grouped based on their microclimate CRID3 sodium salt temperature to control and cold-stressed groups (0 to -8, -8 to -11, and -11 to -14 degrees C). Significant (P < 0.05) decreases in core body temperature and breast and thigh muscle temperatures were observed at simulated transport temperatures below 0 degrees C. In addition, higher (P < 0.05) live shrink and lower blood glucose values were observed as a result of 3-h exposure to temperatures below 0 degrees C, exacerbated as temperature decreased further below -8 degrees

C. Thigh muscle was almost depleted of glycogen reserve compared with a significant but small reduction in breast muscle glycogen when exposure temperature Selleckchem GSK923295 was below -8 degrees C. Similarly, much greater effects were observed on thigh pH(u) and quality attributes compared with breast. In addition, 84% incidence of the dark, firm, dry quality defect was observed in thigh meat (pH(u) > 6.4, L* < 44) compared with 42% incidence of dark, firm, dry in breast meat (pH(u) > 6.1, L* < 46) when transportation

temperature was below 0 degrees C. Results of this study showed that thigh muscle was affected more severely than breast muscle by exposure to cold temperatures before slaughter.”
“Background: This study evaluated the impact of three-dimensional (3D) volume-rendering computed tomography (CT) reconstructions on the inter- and intraobserver reliability of six commonly used classification systems in the assessment of calcaneal fractures. Methods: Four independent observers with different levels of clinical training evaluated 64 fractures according to the classifications of the Orthopaedic Trauma Association (OTA), Essex-Lopresti, Sanders, Crosby, Zwipp, and Regazzoni, using two-dimensional (2D) CT scans with multi-planar reconstructions and 3D volume-rendering reconstructions. Results: Interobserver reliability was moderate for the OTA, Essex-Lopresti, Sanders, Crosby, and Regazzoni classifications with 2D CT scans and 3D CT reconstructions. The Zwipp classification was poor with 2D CT scans and improved to moderate with 3D reconstructions.