In addition, we also confirmed that hirsutanol A could induce autophagical cell death by causing accumulation of ROS level in human hepato cellular carcinoma cells. ROS inducer as selleck chem Regorafenib an antican cer drug has received a lot of attention due to its selective effect on cancer cells but sparing normal cells. To date, there are some ROS inducers targeting ROS generating system or ROS scavenging system. However, most of them cannot enter clinical trials because of the high to icity or poor bioavailability. Here, we reported that hirsutanol A could significant induce cell growth inhibition and apoptosis, elevate the level of ROS in both SW620 and MDA MB 231 cells and sup press tumor growth in SW620 enografts.

Some evidences supported that ROS as a potent o idant agent could damage mitochondrial membrane to result in mitochondrial membrane potential disorder and release of cytochrome c from mitochondria which could further activate caspase 3, leading to mitochon dria cytochrome c mediated apoptosis. We had e amined the mitochondrial membrane potential and the e pression of cytochrome c in mitochondria and cytosol. The results showed that hirsutanol A could trig ger the dysfunction of mitochondrial membrane poten tial and release of cytochrome c from mitochondria. Furthermore, we evaluated whether hirsuta nol A induced growth inhibition and apoptosis were evoked by accumulation of ROS. After treatment with NAC, a potent antio idant agent that could prevent hir sutanol A induced ROS accumulation, we found that cell growth inhibition and apoptosis remarkably decreased.

As our data has clearly demon strated that hirsutanol A could elevate intrinsic ROS level, and activate mitochondria cytochrome c signaliing pathway to trigger apoptosis, further studies are required to elucidate if the release Drug_discovery of cytochrome c is due to the elevated ROS induced by hirsutanol A. ROS, which serves as a second messenger, can modulate several signaling pathways including JNK, Akt, NF ��B etc. In this study, we showed that hirsutanol A enhanced the phosphorylation levels of JNK and c Jun dose and time dependently in SW620 cells. Moreover, preven tion of hirsutanol A induced ROS accumulation by NAC could reverse the phosphorylation of JNK and c Jun. These data indicated that hirsutanol A induced production of ROS activated JNK signaling pathway.

JNK signaling pathway is involved in both stress induced and chemotherapeutical drugs induced apoptosis. However, in hibition of JNK signaling pathway by a special inhibitor SP600125 promoted the hirsutanol A induced cell growth biological activity inhibition and apoptosis. Mass evidences verified that JNK signaling pathway is responsible for regulation of ROS level by activating c Jun, a transcription factor, which further reg ulates the transcription of some target genes involved in redo such as NO and SOD, etc.