Culicoides that inflict biting nuisance have been investigated in greatest detail where they impact tourism, forestry and agriculture ( Hendry, 2011, Hendry and Godwin, 1988 and Linley and Davies,

1971). Despite this record of biting nuisance and their role as vectors of internationally important arboviruses of livestock (Mellor et al., 2000), Culicoides have only rarely been implicated as the primary agents of pathogen transmission to or between humans. Exceptions to this include a range of filarial nematodes transmitted between humans, most notably Mansonella ozzardi, M. perstans and M. streptocerca ( Linley et al., 1983) which are of high prevalence in Latin America and the Caribbean ( Hawking, 1979) and west and central Africa ( Simonsen et al., 2011). Because the clinical Selleckchem Regorafenib manifestation of mansonellosis is commonly Selleckchem Trichostatin A either mild or entirely asymptomatic, examinations of the epidemiology of transmission by Culicoides are relatively rare. A notable exception are the series of detailed investigations

defining relative roles of Culicoides and blackflies (Diptera: Simuliidae) in transmission of M. ozzardi in South America ( Shelley and Coscaron, 2001, Wirth and Felippe-Bauer, 1989 and Yarzabal et al., 1985). By far the most important current role of Culicoides biting midges in public health lies in their ability to biologically transmit Oropouche virus (OROV), the aetiological agent of the febrile illness Oropouche fever, between human beings ( Linley et al., 1983 and Mellor et al., 2000). Commonly observed symptoms of Oropouche fever include headache in a high proportion of cases, but can also lead to generalized arthralgia, anorexia and in rare cases meningitis, the incidence

of which remains undetermined in the vast majority of epidemics ( LeDuc and Pinheiro, 1989). OROV is widely distributed across a geographic range that is thought to include Brazil, Peru, Panama, Colombia and Trinidad ( Karabatos, 1985, Ribonucleotide reductase Nunes et al., 2007 and Saeed et al., 2000), but has not to date been recorded in nearby Costa Rica, Venezuela or other Caribbean islands. Major OROV disease epidemics have largely centered upon Brazil ( Pinheiro et al., 1962, Vasconcelos et al., 1989, Vasconcelos et al., 2009 and Vasconcelos et al., 2011), where thousands of clinical cases can occur and yearly incidence in humans is thought to be surpassed only by dengue among arboviral pathogens, although the lack of specificity of clinical symptoms, combined with a high background of febrile illnesses, hampers accurate reporting.