Amino acid sequences deduced from cDNAs from many genomes have re

Amino acid sequences deduced from cDNAs from many genomes have revealed amino acid sequence homologies in organisms as diverse as bacteria and mammals, particu larly around residues involved in catalysis and metal ion binding. As expected, LAPTc shows the highest identity with the M17 leucyl aminopeptidases Veliparib PARP inhibitor of the kinetoplastids L. major and T. brucei, and less exten sively with the unassigned aminopeptidase II of T. cruzi. Despite conservation of amino acid sequences, M17 members show variable Inhibitors,Modulators,Libraries Inhibitors,Modulators,Libraries pH and temperature optima. Although LAPTc is active over a broad range of tem peratures, its activity shows a marked dependence on neutral pH, since at pH 6 and 8 the enzymatic activity is only 45% of that measured at pH 7. Furthermore, the enzyme is completely inactive at pH 5 and 9.

It should be taken into account that an enzyme may mediate its activity over a broad pH range, Cilengitide depending on the sub strate. Recombinant forms of Leishmania spp. LAPs show optimal activity at pH 8. 0 8. 5 on Leu AMC and have zinc as a cofactor but its 62 kDa monomer does not mediate enzyme activity. The distinguishable features between the two forms of the enzyme might be explained by folding differences, given that rLAPTc was Inhibitors,Modulators,Libraries produced in E. coli and LAPTc isolated from T. cruzi. The higher sensitivity of rLAPTc to SDS is in agreement with this hypothesis. This corre lates well with observations that recombinant members of M17 assemble into active oligomers at 60 70 C and alkaline pHs. Temperatures above 70 C, however, promote inactivation of the thermophilic TAPBb, a member of the M29 family of metallopeptidases, through a transition from the hexameric to the mono meric state.

Since the active form of both endogen ous enzymes lack interchain disulfide bonds, the oligomeric state of LAPTc is even more resistant to high temperatures than that of TAPBb. However, the three dimensional structure of LAPTc seems to unfold at 60 C, the optimal activity temperature of TAPBb. Inhibitors,Modulators,Libraries In spite of displaying leucyl aminopeptidase activity, sequence identity among members of M29 and M17 families is almost absent. Resolution of three dimen sional structures of M29 peptidases may lead to a better understanding of the evolution and activity mechanism of the leucyl aminopeptidase superfamily members. Members of M17 aminopeptidases have a broad range of functional properties beyond the degradation of pep tides.

In animals, plants and bacteria, these enzymes have been implicated in many physiological processes such as protein turnover, regulation of cell redox status, cataract development, MHC I dependent antigen processing and presentation to cytotoxic T cells, nutritional supply, tran scriptional regulation, protein and peptide maturation and defense. A P. falciparum example M17 peptidase is involved in amino acid uptake and regulation and, thus, is considered a virulence factor.

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