A total of 930 μl of TRIS buffer (TRIS 50 mM/EDTA 1 mM; pH 8.2), 4 μl of 30 μM catalase and 50 μl of homogenized tissue or plasmatic supernatant was placed into cuvettes. Then, 16 μl of 24 mM pyrogallol in 10 mM HCl was added to the solution. The sample absorbances were determined in a PF-02341066 manufacturer Lambda 35 spectrophotometer (Perkin-Elmer of Brazil, SP, Brazil), at 420 nm after 60 and 120 s. The results were expressed in SOD units/mg of total protein. Determination of catalase activity (CAT) The CAT activity was determined through the decomposition of hydrogen peroxide at 25°C. In a quartz cuvette, 2865 μl of phosphate buffer 50 mM (pH 7.0)

and 30 μl of homogenized tissue or plasmatic supernatant were added. Then, 35 μl of 0.02 M hydrogen peroxide was added to the solution. The sample absorbances were determined in a Lambda 35 spectrophotometer (Perkin-Elmer Etomoxir solubility dmso of Brazil, SP, Brazil), at 240 nm, and the results are expressed in pmol/mg of total protein [25]. Statistical analysis The data were evaluated using the software Selisistat molecular weight SigmaPlot version 12.0 for Windows. To detect a minimal difference

of 18.91%, with an alpha error of 5% and a power of 80%, the minimal number of animals calculated to be required for each group was ten. This difference was based on a previous study in our laboratory, which utilized an outcome of maximum strength gain (Alves JP, personal communication, 2011). The results were expressed as the mean ± SD. Here, the two-way ANOVA test followed by the Student-Newman-Keuls’ Post Hoc test was used to make comparisons among groups. For associations among variables, the Pearson Correlation Test was performed. The accepted significance level was 5% (P < 0.05). For sample size calculations, the software SigmaPlot version 12.0 for Windows was utilized. To perform correlations and graphics, the software GraphPad 5.0 for Windows was used. Results

The body weight of the animals at the beginning of the study was similar (P > 0.05), but was different by the end. The trained groups demonstrated lower body weight gain when compared to the SED-Cr group (P < 0.01), while the RT group presented lower body weight gain compared to the SED and RT-Cr groups (P < 0.05). Tau-protein kinase Maximum strength gain In relation to absolute maximal strength gain (Figure 1a), a higher strength gain was observed in the creatine supplemented groups and in the group only submitted to RT, compared to the SED group (P < 0.001). The RT-Cr group presented higher maximum strength gain when compared to other groups (P < 0.001). Figure 1 Maximum strength gain after 8 weeks of intervention. a) Absolute maximum strength gain related to the first to fourth tests of One Maximum Repetition (1MR); b) Relative maximum strength gain related to the first to fourth tests of One Maximum Repetition (1MR). Values in mean ± SD; n = 10 for all groups.