2 ml normal saline. After 10 days of challenge the organs (liver and spleen) were aseptically removed and homogenized with a tissue homogenizer (Potter-Elvehjem homogenizer) using 5 ml of saline. The blood (100 μl) was collected from the orbital plexus for HT. The tissue suspensions were transferred to petri-dishes containing the mixture (Sabouraud’s broth + yeast extract + chloramphenicol) and maintained at room temperature for 48 h. The colonies were counted and the number of CFU per organ was determined. Under these conditions the culture gave a yield of approximately 20 × 106 cells per ml and the organisms grew as an essentially pure yeast phase population. The cells were washed twice,

suspended in saline to get desired concentrations and Cisplatin ic50 the viability was checked by methylene blue staining [12]. Statistical analysis.  The statistical analysis of data was done using analysis of variance

(ANOVA) with post-hoc analysis. The Tukey–Kramer post-hoc test was EPZ-6438 in vivo applied to analyze significant changes among groups. The significance of results was ascertained at P < 0.05. All the data are presented as means ± standard error (SE) of the means. GraphPad Prism 5 software (GraphPad Software, Inc., San Diego, CA, USA) was used for statistical analysis. Deltamethrin (18 mg/kg) induced suppression in humoral immunity showing reduction in both parameters, PFC (Fig. 1) and antibody titre (Table 1). Significant (P < 0.001) reduction in PFC was observed in animals treated with deltamethrin alone when compared with control animals (group I). In case of pre and post exposure of fungal infection with deltamethrin the PFC response was significantly (P < 0.001) reduced when compared with the control group.

Infections with C. albicans alone also showed significant (P < 0.001) decrease in PFC response. In case of titre value, there was remarkable decrease in animals treated with deltamethrin when compared with the control group. The titre value in control was 1:682, whereas in DM-treated mice it was 1:64. In animals, treated with fungal infection alone antibody titre was 1:48, whereas it was 1:16 in pre and post exposure of C. albicans with deltamethrin group of animals. Liver of control animals and those treated with deltamethrin Celecoxib alone showed almost no CFU. Animals treated with deltamethrin before as well as after candida infection showed significantly increased number of CFU (Fig. 2). When data of two candida + deltamethrin groups were compared it was observed that animals which were given deltamethrin exposure after C. albicans exposure showed a significantly high number of CFU (P < 0.01). Similar observation was made in case of CFU in spleen (Fig. 3). Deltamethrin appears to be the best compromise between the effectiveness and disadvantages of insecticides, being widely used to control a large variety of agricultural pests and to protect stored products [8, 16, 17].