06 0.71 Fat (g/kg/day) 0.94 ± 0.18 0.97 ± 0.18 0.24 Carbohydrate (g/kg/day) 4.58 ± 1.45 4.32 ± 0.95 0.13 Data are means ± standard deviations of mean. SI unit conversion factor: 1 kcal = 4.2 kJ. Values exclude supplementation dose Statistical Analysis Participant characteristics are reported as means ± SD. All other values are reported as means ± SE. Muscle performance data was expressed as a percentage of baseline values, normalized to the contralateral, undamaged limb. Univariate analysis on the CHO group only was used to examine the effects of the damage

session on muscle performance variables. Differences between the two groups were analyzed using 2 × 7 (group × time [Day 1, 2, 3, 4, 7 10 and 14) repeated measures analysis of variance (ANOVA) to effectively assess the changes in muscle function/strength following supplementation post-exercise. Blood variables were analyzed using 2 × 14 (group × time [baseline, 30 min, PD0325901 cost 60 min 2 hours, 4 hours, day 1, 2, 3, 4, 7 10 and 14) repeated measures ANOVA to effectively assess the changes in markers of muscle damage following supplementation post exercise. Least significant difference Romidepsin in vivo pairwise comparisons was used to analyze any significant group × time interaction effects.

Baseline variables, total work performed during the resistance exercise session and dietary intake between groups were analyzed using a students’ t-test. An alpha level of 0.05 was adopted throughout to prevent any Type I statistical errors Results Participant Characteristics At baseline there were no differences in the age, body weight or strength level (1RM) between the two groups (see Table 1). Total lifting Volume During the resistance training session, the number of repetitions and weight lifted (120% of 1RM) was recorded for each exercise. Total lifting volume for each group reflects the total number of repetitions multiplied by the total

weight lifted performed by each participant for each exercise (see Table 3). No differences were detected between groups. Table 3 Total Lifting Volume Characteristics CHO WPH P-value Leg Press 1RM (kg) 18000 ± 7344 18576 ± 5760 0.11 Leg Extension 1RM (kg) 12672 Immune system ± 3744 12096 ± 3600 0.49 Leg Flexion 1RM (kg) Extension 5760 ± 1152 6624 ± 3168 0.60 Data are means ± standard deviations of mean. SI unit conversion factor: 1 kg = 2.2 lbs Dietary Analysis One-week dietary analysis (excluding supplementation) revealed no differences in energy, protein, fat and carbohydrate intake between groups throughout the study (see Table 2). Based on supplement dosage of 1.5 g/kg.bw/day, there was no difference in the amount of supplement ingested between the CHO and WPH supplemented groups during the 14-day recovery period. Isometric Knee Extension Strength Pre-exercise absolute values for isometric knee extension strength were 314 ± 27 Nm and 290 ± 17 Nm for CHO- and WPH-supplemented groups, respectively, and were not significantly different.