Moreover, this was associated with a significant increase of the expression of upstream Wnt1, consistent with the up-regulation of lower-stream CyclinD1 and c-Myc at protein level (Figure 5B). Figure 5 Wnt/β-catenin was up-regulated in tumors derived from SP cells.(A) Quantitative RT-PCR analysis revealed that the expression of β-catenin, TCF4, LEF1, CyclinD1 and c-Myc (mean ± SD) were higher in tumors derived from SP than those in tumors from non-SP. These differences were all statistically significant (* P < 0.05, ***P < 0.001).

(B) Western blotting analysis find more showed that Wnt1, β-catenin, CyclinD1 and c-Myc in tumors derived from SP expressed higher than those in tumors from non-SP cells. The experiment was run in triplicate. The effect of CKI on SP cells in vivo Tumor volumes were measured for up to 7 weeks after inoculation (Figure 6A). Incised tumors

among three groups were compared (Figure 6B). Both the CKI and DDP groups showed lower tumor formation rates compared to the SRT2104 supplier control group (P < 0.05) (Figure 6C). A representative mouse specimen without a tumor was observed in the CKI group (Figure 6D), whereas a representative specimen with a tumor was observed in the control group Ferrostatin-1 research buy (Figure 6E). No body weight loss was observed in the CKI group, whereas a slight body weight loss was observed in the DDP group (Figure 6F). Figure 6 In vivo efficacy of CKI in the MCF-7 SP xenograft model. (A) Tumor volumes (Mean ± SEM) were plotted for each group (n = 6 per group). Both CKI and DDP suppressed Casein kinase 1 tumor growth. (B) A representative comparison image

of the incised tumors from CKI, DDP, and the control group. (C) The tumor formation rate of the control group was 100% (6/6), while that of CKI group was 33.33% (2/6) and that of the DDP group was 50% (3/6) (* P < 0.05). (D) A representative mouse specimen without a tumor from the CKI group. (E) A representative specimen with a tumor from the control group. (F) Schematic outline of mice body weight (mean ± SD). No body weight loss was observed in the CKI group, but a slight body weight loss was observed in the DDP group compared to the control group. Canonical Wnt/β-catenin pathway analysis on CKI and DDP group in vivo Western blot and RT-PCR analyses were used to investigate whether CKI could down-regulate the expression of the main components of Wnt/β-catenin Pathway. The study found a dramatic decrease of β-catenin with CKI treatment, but the same down-regulation was not observed at the mRNA level.