The consumption of beverages from unreliable sources, containing higher concentrations of methanol, has been responsible for severe poisonings leading to central nervous system disorders, particularly blindness, and even death ( Badolato

& Duran, 2000). Especially dangerous are cachaças to which illicit additions of ethanol used as fuel were made, since it may had been adulterated with methanol ( Carneiro et al., 2008). There are several analytical methods described for the detection and quantification of methanol in the presence of ethanol being chromatography (Wang et al., 2004 and Zenebon et al., 1996) the most common. Other techniques are, for instance, surface plasmon resonance (SPR) (Manera et al., 2004), multi-enzyme system with chemiluninescence detection (Sekine, Suzuki, Takeuchi, Tamiya, & Karube, 1993), Fourier Transform Infrared Spectrometry (Bangalore, Small, Combs, Knapp, & Kroutil, Ibrutinib ic50 1994), and whole-cell biosensing (Naessens & Tran-Minh, 1998). These methods are expensive or need to be performed in a laboratory, normally far from the site where the analysis is needed. The development of chemiresistors sensitive to organic vapours, based on metal-oxide semiconductors (MOS) (Gardner and Bartlett, 1999 and Stephan et al., 2000), MOS field-effect

transistors (MOSFET) (Naessens and Tran-Minh, 1998 and Gardner and Bartlett, 1999), and electrically conductive polymers has been described (Benvenho et al., 2009, Gardner and Bartlett, 1999, Gruber et al., 2004, Li et al., 2009, Li et al., 2008, Péres and Gruber, 2007, Rosa AZD9291 purchase et al., 2005 and Vanneste

et al., 1998). The advantages of the latter are that they operate at for room temperature with very low power consumption, do not require expensive equipment and are portable. In the particular case of methanol vapours the sensors described so far are based on MOS (Bangalore et al., 1994 and Patel et al., 2003) and do not show any selectivity towards methanol when mixed with ethanol. In the present work, we describe a low-cost, rapid and accurate method for the determination of methanol in cachaça, based on a chemiresistive polymeric gas sensor, whose active layer is a thin film of a conducting polymer, poly(2-dodecanoylsulfanyl-p-phenylenevinylene) (12COS-PPV) ( Scheme 1), doped with camphorsulfonic acid. Since the sensor is sensitive to methanol, but not to ethanol, it can be used for detecting methanol in cachaça or in any other alcoholic beverage. Poly(2-dodecanoylsulfanyl-p-phenylenevinylene) (12COS-PPV), was synthesised from commercial 2,5-dimethylbenzenethiol (Aldrich, 98%) in three steps as previously described in the literature ( Gruber et al., 2004). The polymerisation step was carried out electrochemically ( Utley & Gruber, 2002) at a controlled potential of 1.41 V vs. Ag/AgBr.