Critically, the Memory × Region interaction was also significant

Critically, the Memory × Region interaction was also significant (left: F(1,29) = 39.20, p < 0.001; right: F(1,29) = 36.6, p < 0.001), indicating that the effect of Memory significantly differed across regions. We then analyzed each region separately. Of course, there was a significant main effect of Memory in IPL (left: F(1,29) = 47.88, p < 0.001; right: F(1,29) = 34.97, p < 0.001). The main effect of Memory in IPS was not significant (left: F(1,29) = .98, p = .33; right: F(1,29) = 2.56, p = 0.12). The Region × Attention × Memory interaction was not significant

(both hemispheres: F ≤ 1). These analyses indicate that the dissociation between the IPS and the IPL does not depend on the threshold employed in the whole-brain analysis. The interaction between visual attention and episodic retrieval is poorly learn more understood. Given that the neural systems mediating attention and episodic memory appear to be anatomically segregated, and perhaps even in competition, it is unclear which neural systems are engaged http://www.selleck.co.jp/products/Rapamycin.html when visual attention is recruited during episodic retrieval.

We investigated the recruitment of visual attention by episodic retrieval during the suppression of gist-based false recognition. When two similar candidate targets were presented next to each other, participants had to systematically compare the two items and attend to the details that distinguished them in order to decide whether one of the items was old (Attention-High conditions). This process was associated with increased activity in regions previously associated with top-down visual attention ( Kastner and Ungerleider, 2000; Corbetta and Urease Shulman, 2002), including the IPS ( Figure 2). These results suggest

that systems for top-down visual attention, although not typically associated with episodic retrieval, can play an important role when retrieval of specific visual details is required. Although activity in the IPS was associated with the attempt to retrieve perceptual detail, it was not associated with successful retrieval of perceptual detail. In contrast, activity in the IPL, and other regions likely overlapping with the default network, was associated with the successful retrieval of perceptual detail from memory ( Figure 4). Thus, the IPS and the IPL make dissociable contributions to the retrieval of perceptual detail. Below, we discuss the implications of these findings for models of the role of the parietal cortex in episodic retrieval and visual attention. When two candidate targets were presented adjacent to one another (Attention-High conditions), participants had to systematically compare the two candidate targets and attend to the details that distinguished them in order to decide which item was old.

The diet journal prompts users to enter the following information

The diet journal prompts users to enter the following information upon consumption: time of entry, type VX-770 datasheet of food/drink, amount, unit, brand, and way of preparation. The researchers provided detailed instructions on how to document nutrition information. When difficulty arose in determining portion sizes, the participants were advised to use the following methods: (a) reading food or beverage labels; (b) referring to the examples provided in the diet journal; and (c) seeking help from adults or more knowledgeable others. At the end of the study, a trained data analyst entered

the data from each diet journal into Nutritionist Pro (Axxya Systems™, Stafford, TX, USA), which generated EI data (in kcal). The Nutritionist http://www.selleckchem.com/products/epz-6438.html Pro was selected for

diet analysis because of its comprehensive database, high efficiency of the search engine in finding foods, adaptable output feature, and affordable cost.24 The above method to measure EI was previously used and was found accurately estimating EI among a sample of overweight/obese adults.16 EE was measured by the SWA. SWA is a non-invasive, wireless multi-sensor monitor worn on the left triceps using an adjustable strap. It relies on several parameters (i.e., heat flux, galvanic skin response, skin temperature, near body temperature, and motion being determined by a tri-axial accelerometer) to measure EE (in kcal), time spent in PA of various intensities (in minute), and other movement outcomes. The SWA is user-friendly

and has showed sound criterion validity and test retest reliability for assessing free-living EE.25 Weight was measured using a digital weight scale (Tanita HD-366; Tanita, Arlington Heights, IL, USA). The scale took measurement of weight in kilogram, which was also converted to pound and ounce. Height was measured by the Seca 213 stadiometer (Seca™, Phosphoprotein phosphatase Hanover, MD, USA). The stadiometer provided measurement in centimeter. BMI was calculated to adjust natural body growth. The estimated EB was obtained by calculating the difference between EE and EI; while actual EB was obtained through a mathematical conversion from body weight. Because one pound of body fat can be deemed as 3500 kcals of energy, actual EB was computed by multiplying the weight changed (in pound and ounce) and 3500.6 The data collection procedure was carried out step by step as described below. During the first school visit, class rosters were obtained from the PE and/or health teachers. Because there were a number of students per class participated in this research project, the class rosters were reduced with participants who were coded by de-identified numbers. The participants were randomly assigned by the lead author into the experimental or the control groups. Overall, each class had roughly even number of participants per group. During the second school visit, the participants were informed of their group assignment (i.e., experimental or control group).

The green leaves were dried in a stove with hot air circulation a

The green leaves were dried in a stove with hot air circulation and thermostatized

at 40 °C during 4 days. After grinding them, the resulting material was subjected to extraction with ethyl acetate and ethanol (3:1), using 9 L of this mixture for each extraction. This process was repeated four times with an interval of 7 days between extractions. The total weight of the extract obtained corresponded to 5.5% of fresh plant mass ( Cotinguiba et al., 2009). The essential oils from L. sidoides CH5424802 research buy and M. piperita were obtained at the Embrapa Western Amazon Research Station from plants cultivated in Manaus, Amazonas state, Brazil. The leaves of L. sidoides and M. piperita were cut at ground level and placed in a freezer until extraction. After separation of the leaves, two samples of 20 g were used to determine moisture

by drying an oven at 65 °C for 3 days. Two other samples of 100 g each were used to extract the essential oil by hydrodistillation in a Clevenger type apparatus for 3 h. H. crepitans latex was collected in the trees located in the city of Porto Velho, Rondônia state by employees of Embrapa Rondônia. The seed oil of C. guianensis was produced and acquired in the local market of Porto Velho. The active substances from P. tuberculatum used in the present trial were previously described by Cotinguiba et al. (2009). Chemical analyses of the L. sidoides and M. piperita essential oils, C. guianensis oil and H. crepitans latex were performed by the Embrapa Food Agribusiness Research Unit (CTAA). The identification of the essential oil components BMN 673 mouse was carried out by gas chromatography coupled to mass spectrometry (GC-MS) in an Agilent 5973N system (Agilent Technologies, Delaware, USA) equipped with an HP-5MS capillary column (5% diphenyl, 95% dimethylsilicone, 30 m × 0.25 mm; film thickness 0.25 μm). Helium was used as the carrier gas (1.0 mL min−1), with injection of 1.0 mL of a 1% solution of the essential

oil in dichloromethane in an injector heated to 250 °C, operating in split mode (split ratio 1:100). either Oven temperature was varied from 60 to 240 °C at a rate of 3 °C min−1. The mass detector was operated in electron ionization (70 eV) with the mass analyzer maintained at 150 °C, the ionization source at 220 °C and transfer line at 260 °C. To obtain the quantification, the essential oils were also analyzed in an Agilent 7890A chromatograph (Agilent Technologies, Delaware, USA) equipped with a flame ionization detector (FID) kept at 280 °C and fitted with an HP-5 capillary column (5% diphenyl–95%-dimethyl silicone; 30 m × 0.32 mm; film thickness 0.25 μm). The same injection and chromatographic conditions above were applied, but hydrogen was used as the carrier gas, at 1.5 mL min−1. The results were indicated through relative area (% area). Linear retention indices were calculated by injection of a series of n-alkanes (C7–C26) in the same column and conditions stated for GC-FID analyses.

As expected for a KATP-mediated current, current “run-up” was abs

As expected for a KATP-mediated current, current “run-up” was absent in Kir6.2−/− neurons or in wild-type neurons that were incubated in 200 μM tolbutamide prior to recording ( Figure 5C). To test for a whole-cell correlate of the high resting single channel Popen observed in cell-attached

patches, we performed experiments with high ATP (4 mM) in the pipette, with the idea that as the ATP washes into the cell it might inhibit any initially active KATP channels. Indeed, we found that conductance in Bad−/− neurons decreased INCB28060 in vivo within the first minute after break-in with high ATP and then remained constant ( Figure 5D). This “washdown” was not seen in wild-type cells ( Figure 5D). It was also eliminated in Bad−/− neurons if they were preincubated with tolbutamide ( Figure 5E) or in neurons from animals that lacked both BAD and Kir6.2 ( Figure 5D), confirming that this initial high conductance was due to KATP channels. Current washdown also occurred in neurons from BadS155A mice ( Figure 5D),

showing that it is BAD’s metabolic, rather than apoptotic, function that is responsible for increasing KATP conductance. The marked increase in KATP channel open probability in Bad−/− DGNs suggests a link between KATP channel conductance and seizure protection in these mice and predicts that BAD’s effect on seizure sensitivity may be mediated by the KATP channel. To test this prediction, we generated Bad−/−; Kir6.2−/− double mutant mice and tested their sensitivity to KA in parallel with single Bad−/− or Kir6.2−/− mutants. Ablation of the selleck inhibitor Kir6.2 subunit in the Bad null

genetic background substantially diminished the seizure resistance phenotype of Bad−/− mice ( Figures 6A and 6B). Single deletion of the Kir6.2 subunit did not sensitize Thymidine kinase mice to acute seizures, arguing against an orthogonal or additive effect on seizures. These findings provide genetic evidence that the KATP channel is required for mediating BAD’s effect on neuronal excitability. Using a combination of genetic models and multiple experimental approaches ranging from mitochondrial respirometry in primary neural cultures and slice electrophysiology to behavioral and electrographic seizure monitoring in vivo, we provide a vertical analysis of BAD’s effect on neural carbon substrate utilization, neuronal excitability, and seizure susceptibility. Our observations suggest that BAD imparts reciprocal effects on glucose and ketone body consumption through a phosphoregulatory mechanism that modifies S155 within its BH3 domain. Specifically, BAD deficiency or interference with its phosphorylation is associated with diminished mitochondrial metabolism of glucose and a concomitant metabolic preference for ketone bodies. An electrophysiologic consequence of this metabolic shift is a marked increase in the open probability of the metabolically sensitive KATP channel.

These data show that the Ca2+-CaM dependent Munc13-1 mediated rep

These data show that the Ca2+-CaM dependent Munc13-1 mediated replenishment of the rapidly releasable SV pool does not significantly affect SSD levels in young calyx of Held synapses. Because the relative contribution of mechanisms that define the steady-state EPSC amplitudes during train stimulation changes during postnatal maturation of the calyx (Crins et al., 2011; Erazo-Fischer et al., 2007; Sonntag et al., 2011; Taschenberger et al., 2002, 2005; Taschenberger and von Gersdorff, 2000; Wang et al., 2008), we tested whether STD differs between more mature WT and

Munc13-1W464R synapses. We measured SSD levels during trains of 25 APs at frequencies of 2–100 Hz in P14–P17 calyces. SSD levels in calyces of Munc13-1W464R Fulvestrant supplier mice this website were significantly lower than those of WT mice at all frequencies tested (Figures 7A–7C, S3E, and S3F), whereas the initial EPSC amplitudes were unchanged (100 Hz train; WT, 20.55 ± 2.3 nA, n = 16; Munc13-1W464R 24.3 ± 3.02 nA, n = 17; p > 0.05). The stronger SSD in P14–P17 Munc13-1W464R KI calyces was accompanied by significantly smaller PPRs in Munc13-1W464R mutants as compared to WT animals (Figure 7D). Presynaptic Ca2+ current amplitudes (WT, 1.85 ± 0.2 nA, n = 5; Munc13-1W464R, 1.96 ± 0.3 nA, n = 6; p > 0.05),

and facilitation of the Ca2+ current during trains of step depolarizations were similar in Munc13-1W464R and WT calyces (Figures 7E–7G), and therefore cannot account for the differences observed in pr. These data demonstrate that genetic perturbation of Ca2+-CaM signaling to Munc13-1 results in aberrant STD nearly in the calyx of Held after hearing onset, but not at calyces

of juvenile mice. STD during high-frequency AP trains is a feature of many synapses in the mammalian brain, including the calyx of Held (Figures 6 and 7). It primarily reflects a transient and activity dependent decrease in neurotransmitter release, which can be caused by several different processes, including reduced Ca2+ influx into presynaptic terminals (Xu and Wu, 2005), changes in the AP waveform (Geiger and Jonas, 2000), depletion of the RRP of SVs (Rosenmund and Stevens, 1996; Sakaba and Neher, 2001; Wu and Borst, 1999), and delayed clearance of SV release sites (Hosoi et al., 2009). STD is counteracted by the SV priming machinery, which consists of Munc13 and CAPS proteins and determines the rate of RRP refilling and the RRP size after strong stimulation (Augustin et al., 1999b; Jockusch et al., 2007; Junge et al., 2004; Rhee et al., 2002; Rosenmund et al., 2002; Varoqueaux et al., 2002).

Metronidazole was once considered to be teratogenic, however 50 y

Metronidazole was once considered to be teratogenic, however 50 years of usage has quelled that concern. However, treatment of Tv during pregnancy did not have the impact of reducing pregnancy complications as hoped. Metronidazole treatment during pregnancy was found to increase preterm labor (relative risk 3.0) compared to placebo (untreated) Tv infections [24] and [25]. A potential conflicting factor of the results from the Klebanoff

study is a nonstandard metronidazole dosage regime. Yet while no evidence of direct causality has been reported, it is speculated that dying Tv find more or the release of virus contained in some strains of Tv may result in stimulation of innate immune response or changes in bacterial flora that affect the pregnancy outcome, but studies are required to confirm this [25]. The overall data regarding Tv infection and pregnancy strongly suggests the value of screening

and treatment of women seeking to become pregnant, or are at risk of unplanned pregnancies, and their male partners. Reports regarding the increased transmission EGFR targets and acquisition of HIV in Tv infected study participants has stimulated recent interest in the parasite. The odds ratio of a female with Tv acquiring HIV has been measured between 1.52 and 2.74 [10], [26] and [27]. A mathematical model of HIV infection based on a 1.8 odds ratio of acquiring HIV when infected with Tv estimates that 2% of all HIV acquired by females in the United States may be attributable to Tv [28]. In regions where Tolmetin Tv is more prevalent such as in Africa, the impact of Tv on HIV transmission could be higher. Guenthner and colleagues [29] investigated the ability of HIV-1 to pass through a polarized monolayer of epithelial cells in conjunction with Tv. They demonstrated p24 gag could be detected in the basolateral supernatant in greater quantities

compared to controls without Tv. Furthermore, differences in amount of epithelial damage based upon the Tv isolate was positively associated with HIV-1 passage through the monolayer. An additional experiment investigated the ability of Tv-stimulated peripheral blood mononuclear cells (PBMC) acutely infected with HIV-1 to induce replication of HIV-1. Activation of the acutely infected PBMC promoted HIV-1 replication. Thus two proposed mechanisms of synergy of Tv and HIV-1 were the pathogenesis of the Tv isolate’s ability to induce damage to epithelial cells and the activation of acutely infected PBMC [29]. The relationship of Tv and HIV is reviewed in more detail elsewhere [30]. Co-infection of Tv and HIV in men and women is positively associated (odds ratio of 1.22 and 1.31, respectively) [31] with further reports identifying more Tv infections in HIV+ than HIV− patients and an odds ratio of 2.12 for HIV+ individuals to acquire Tv [26] and [32]. Lower CD4 inhibitors counts (40–140 and 150–250 cells/mL) and higher viral loads have been reported to be associated with likelihood of Tv diagnosis [33] and [34].

Therefore, the development of a vaccine to prevent Trichinella in

Therefore, the development of a vaccine to prevent Trichinella infection in domestic learn more animals and humans is a necessary approach for controlling this disease. Heat shock proteins (Hsps) are a group of proteins that are induced upon exposure to a range of environmental stresses that include heat shock, oxygen deprivation, pH extremes, and nutrient deprivation

[6]. This Modulators family of proteins is highly conserved among different species and highly immunogenic during infections [7], [8], [9] and [10]. The heat shock proteins have recently been reported to play significant roles in antigen presentation, the activation of lymphocytes, and the maturation of dendritic cells [11]. Several researchers have also reported on the protective efficacies of Hsps against various infections by Plasmodium yoelii [7], Brugia malayi [8], Leishmania donovani [9], and Hantaan virus [12]. Several RAD001 purchase heat-shock proteins, such as Hsp60, Hsp70 and Hsp80, have been reported and named according to their molecular weight. Of these proteins, Hsp70 is the

most conserved among different organisms, and Hsp70 is an immunodominant antigen during infections caused by a number of pathogens [6], [13] and [14]. In our previous study, Hsp70 from Trichinella spiralis (Ts-Hsp) was cloned via the immunoscreening of a T. spiralis cDNA library with immune serum, and the recombinant Ts-Hsp70 protein (rTs-Hsp70) was expressed in an Escherichia coli expression system [15]. The rTs-Hsp70 protein was recognized not only by the sera from patients with trichinellosis but also in the sera from T. spiralis-infected rabbits, pigs, and mice. The native Ts-Hsp70 was found in the crude somatic extracts of T. spiralis muscle larvae and adult worms. Vaccination with rTs-Hsp70 induces a strong immune response and a 37% reduction in muscle

larvae upon T. spiralis larval challenge compared to PBS control groups [15]. Further investigations in our lab demonstrated that the immunization of mice with rTs-Hsp70 elicited a systemic Th1/Th2 immune response (data not shown). However, as a possible vaccine candidate antigen, the mechanism of Ts-Hsp70-mediated protection of requires further clarification. One mechanisms by which an antigen is presented to the immune system is based on the antigen’s ability to alter the maturation of dendritic cells (DCs). DCs are the typical antigen presenting cells (APCs) that induce primary immune responses through the activation and differentiation of helper T cells [16] and [17] and play a crucial role in helminth infections [18] and [19]. Currently, it remains unclear whether the protective immune response against T. spiralis infection induced by rTs-Hsp70 is related to DC activation. In this study, the interaction between rTs-Hsp70 and DCs derived from mouse bone marrow was investigated.

Their EGFR as

Their MLN8237 in vitro baseline characteristics are presented in Table 1. Ten (53%) participants undertook the control intervention (exercise using either a treadmill or cycle ergometer as prescribed by the treating physiotherapist) first. The two exercise

interventions were conducted for all participants within a 48 hour period, within 72 hours of discharge. Both exercise modes were delivered by the same physiotherapist in the Physiotherapy Gym of the Adult Cystic Fibrosis Unit at The Prince Charles Hospital in Brisbane, Australia. Exercise heart rate and oxygen saturation data inhibitors during rest and each exercise intervention are presented in Table 2. During the 15-minute exercise, there was no significant difference in the average heart rate between the gaming console exercise of 144 beats/min (SD 13) and control exercise of 141 beats/min (SD 15), mean difference 3 beats/min (95% CI −3 to 9). However, gaming console exercise induced a significantly higher maximum heart rate, by 9 beats/min (95% CI 3 to 15) and a significantly higher minimum heart rate, by 13 beats/min (95% CI 2 to 24). Average, maximum and minimum oxygen saturation during exercise did not differ significantly

between the groups, with between-group differences of only 1–2% (absolute). Participants thought both exercise modes provided a ‘hard’ workout, rating each on average a score of about 15 on the RPE AZD6738 mouse scale (Table 3). Energy expenditure at rest and during the 15 minutes of exercise is presented in Table 2. No data were recorded for two participants, one each in both exercise interventions. There were no significant differences between the two exercise modes during the 15 minutes of exercise (1.0 MET, 95% CI −0.3 to 0.5). However, there was a significant difference between the two exercise interventions for the total energy expended in the whole exercise session before (26 kcal, 95% CI 17 to 35), as presented in Table 3. The participants’

perception of the exercise is presented in Table 3. Participants rated the gaming console exercise as significantly more enjoyable on the 10-cm visual analogue scale, mean difference 2.6 cm (95% CI 1.6 to 3.6). Participants did not perceive significantly different fatigue or workload between the two types of exercise. Participants thought both exercise modes were an effective form of exercise, rating each on average a score of about 8 on the visual analogue scale. Similarly, participants thought both exercise modes would be feasible to include as part of their regular exercise regimen, rating each on average a score of about 8 on the visual analogue scale. The amount of dyspnoea also did not differ between the two types of exercise. Exercise involving a gaming console appears to be a feasible mode of aerobic exercise for adults with cystic fibrosis.

, 2008) Together, these observations strongly suggest that ATP i

, 2008). Together, these observations strongly suggest that ATP is localized in secretory acidic vesicles in inhibitors cultured Müller cells. Moreover, together with the observation that Evans blue blockade of quinacrine staining was reversible, our results also suggest that cultured avian Müller cells store ATP in acidic vesicles through the functioning of VNUT or a related vesicular anion transporter sensitive

to Evans blue. One interesting point to be further explored is whether cultured Müller cells express this or some other similar transporter. One major role of Müller glial cells is to regulate the composition of the retinal extracellular fluid. Neuronal activity results in increases in extracellular K+ in the inner and outer plexiform layers and these variations http://www.selleckchem.com/products/AC-220.html lead to an influx of K+ into Müller GS 1101 cells by a spatial-buffering mechanism, also known as “K+ siphoning”, that depolarizes glial cells (Newman and Reichenbach, 1996). Moreover, Müller cells express voltage-dependent calcium channels (Newman, 1985) that were characterized as L-type of calcium channels in the

human retina (Puro et al., 1996). Accordingly, high concentrations of extracellular K+ can induce an increase in intracellular calcium levels (Keirstead and Miller, 1995 and Wakakura and Yamamoto, 1994). In the present work, we show that incubation of chick Müller glial cells with a 50 mM solution of KCl induced

both a decrease in quinacrine staining of cell vesicles and a significant accumulation of ATP in the culture medium, suggesting that under depolarization, cultured Müller glia cells release ATP through the exocytosis of nucleotide-filled vesicles. Although ATP release from glial cells can occur by many different pathways, such as aminophylline connexin hemichannels (Stout et al., 2002), purinergic P2X7 receptor (Anderson et al., 2004) and ATP transporter proteins (Abraham et al., 1993), the release of ATP by exocytosis was demonstrated in astrocytes (Bal-Price et al., 2002, Coco et al., 2003 and Pangršič et al., 2007) and Schwann cells (Liu et al., 2005). Müller glial cells express several glutamate receptors, including NMDA, AMPA/KA and metabotropic glutamate receptors (Keirstead and Miller, 1997, Lamas et al., 2005, López et al., 1994, López et al., 1997, López-Colomé and Romo-de-Vivar, 1991, Uchihori and Puro, 1993 and Wakakura and Yamamoto, 1994). As for KCl-mediated depolarization, incubations with glutamate induced a decrease in quinacrine staining as well as an increase in extracellular ATP content in retinal Müller cells in culture (Fig. 4 and Fig. 5).

Predominantly white, the area is characterized by high rates of u

Predominantly white, the area is characterized by high rates of unemployment, poverty, and chronic disease. Data collection took place from February-August, 2011, in two Women, Infants, and Children (WIC)4 clinics (USDA, 2011). These two sites were selected because they served the largest proportion of low-income residents in the region. In LA County, CPPW funded interventions for 9.8 million adults and children countywide. LA County is largely urban with a land area of 4058 square miles and a population density of 2419 persons per square mile. The population is racially and ethnically diverse.

LA County is similar to WV in that its northwest and south-central regions have high rates of poverty and chronic disease (Los Angeles County Department of Public Health (LACDPH), 2011 and U.S. Census Bureau (QF-L), 2012b). Data were collected from http://www.selleckchem.com/products/gsk-j4-hcl.html February–April, 2011 in five Birinapant in vivo public health centers operated by the Los Angeles County Department of Public Health (LACDPH).5 These centers provide a range of services (e.g., immunizations,

treatment of tuberculosis and sexually transmitted diseases, community programming, and other public/social services) to low-income residents. We selected them because they are located within the most impoverished areas of the county. WV participants (total n = 630; women with children ages 0–5 years, n = 553) were recruited from the waiting rooms of the two selected WIC clinics. To be eligible, they had to

meet the following criteria: 1) demonstrated interest in the project; 2) be at least 18 years of age; 3) read and spoke English; 4) lived in one of six county jurisdictions in WV; 5) not be pregnant; 6) be at least eight weeks postpartum; and 7) agreed to return for follow-up visits (i.e., at three and six months post-initial encounter). All WIC clients those had incomes that fell at or below 185% of the U.S. Poverty Income Guidelines (USDA, 2003). In LA County, low-income participants (total n = 720; women, n = 408) were recruited from the waiting rooms of five large public health centers using a Modulators systematic approach to selection, accounting (when feasible) for each center’s clientele volume, time of day, variation in the types of services provided, and variation in clinic flow on the specified recruitment days. Trained staff utilized multi-stage, systematic procedures on pre-specified days of the survey period to recruit and enroll eligible participants. To be eligible, LA County participants had to meet the following criteria: 1) be at least 18 years of age; 2) spoke English or Spanish; 3) be a client (patient) of the health center; 4) not be pregnant; and 5) agreed to complete a battery of anthropometric and self-administered assessments on a scheduled weekend day at a designated center location. Standardized recruitment and measurement protocols were used in both communities.